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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recombinant, human p34Cdk1/cyclin B expressed in S. frugiperda insect cells. This kinase is a major regulator of the eukaryotic cell cycle. As a monomer, Cdk1 is inactive and requires association with cyclin B and phosphorylation of Thr161 to form an active complex. MW: 34000(p34Cdk1) and 55000(cyclinB).
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM NaCl, 50 mM HEPES, 1 mM DTT, 100 µM EDTA, 50% glycerol, 0.01% BRIJ® 35 Detergent, pH 7.5. | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit is defined as the amount of enzyme that catalyzes the transfer of 1 pmol of phosphate to histone H1 (10 µM) in 1 min at 30°C, pH 7.5. | ||
| Activity | ≥20,000 U/ml | ||
| Contaminants | Protease, phosphatase: none detected | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
||
