70181
NovaBlue Singles™ Competent Cells
High-efficiency transformation of E. coli in less than 8 minutes
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
23 May 2013
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NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-µl volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance).
Genotype:endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)
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| 71408 | Origami™ 2(DE3) Singles™ Competent Cells |
| Product information | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Avoid freeze/thaw | Yes | |||||||||||
| Components |
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| Genotype | endA1 hsdR17 (rK12– mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR) | |||||||||||
| Guaranteed efficiency | Guaranteed efficiency: > 1.5 x 108 cfu/µg | |||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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