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70181  NovaBlue Singles™ Competent Cells

High-efficiency transformation of E. coli in less than 8 minutes

For general questions please contact our Customer Service:

EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 800-645-5476
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com

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20 April 2014

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Product number Qty/Pk Quantity Price Available to Ship
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70181-3  11 rxn 
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70181-4  22 rxn 
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NovaBlue Singles Competent Cells, like all Novagen Singles Competent Cells, are designed for ultimate convenience and reliability in plasmid transformation. The cells are grown and made chemically competent by an optimized procedure. Cells are provided in 50-µl volumes that eliminate the need to aliquot, freeze/thaw, or waste partially used vials, thus saving time and increasing performance. For use, simply thaw, add DNA, incubate five minutes on ice, heat shock for 30 seconds, place on ice for two minutes, add SOC Medium, and plate directly (when selecting for ampicillin resistance) or after incubation at 37°C for 30 minutes (when selecting for kanamycin resistance).

Genotype:
endA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)
Product information
Avoid freeze/thaw Yes
Components
11 or 22Singles Competent Cells
2 or 4 × 2 mlSOC Medium
10 µlTest Plasmid
Genotype endA1 hsdR17 (rK12 mK12+) supE44 thi-1 recA1 gyrA96 relA1 lac F′[proA+B+ lacIqZΔM15::Tn10] (TetR)
Guaranteed efficiency Guaranteed efficiency: > 1.5 x 108 cfu/µg
Store and ship information

Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.

Storage ≤ -70°C
Ship Dry Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2014

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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