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NanoJuice® Transfection Reagent Kit

 

 

  

There is no shortage of methods for introducing plasmid DNA into mammalian cells: DEAE dextran, calcium phosphate coprecipitation, lipofection, microinjection, electroporation….just to name a few. Unfortunately, these traditional methods have limitations, including low transfection efficiency and high cytotoxicity. In addition, many cell types – primary cell lines in particular – stubbornly resist transfection. It is challenging to find one transfection reagent that is flexible enough to work with diverse cell types. Ideally, you could use a single transfection kit to optimally transfect a variety of cell types, including the recalcitrant ones. We designed the NanoJuice® Transfection Kit for this purpose.

NanoJuice® Transfection Kit

The NanoJuice® Transfection Kit is designed for efficient transfection of difficult-to- transfect cells, such as primary cells, Saos-2, Caco-2, HUVEC, Jurkat, Raw 264.7, HeLa, and other mammalian cells. The NanoJuice reagent mixture uses the cutting-edge nanotechnology of Priostar® dendrimers along with a proven polycationic liposomal formulation. This combination of NanoJuice Core Transfection Reagent and NanoJuice Transfection Booster allows you to fine-tune transfection conditions for each specific cell type. Complete control over transfection conditions allows you to optimize transfection efficiencies and reduce cytotoxicity.

Kit Features:

  • Suitable for stable and transient transfections
  • Minimal cellular toxicity
  • Compatible with both serum-containing and serum-free media
  • Allows flexible reaction conditions to optimize for specific cell types
  • Eliminates the need for media changes
  • Derived from non-animal sources
  • Based on the cutting-edge nanotechnology of Priostar dendrimers

© Merck KGaA, Darmstadt, Germany, 2013

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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