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70969  Mobius™ 500 pET Plasmid Kit

“Maxi” scale configuration for purification of pET plasmids

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25 May 2012

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The Mobius™ 500 pET Plasmid Kit is a special configuration Mobius kit designed for "maxi" scale preparations of pET and other plasmids having a low-copy (≤ 40 copies per cell) origin of replication. Up to 500 µg ultrapure plasmid can be isolated from 500 ml bacterial culture using Mobius anion exchange chromatography. Columns, buffers, and purification protocol are optimized for the larger-scale cultures required for low-copy plasmids.


Product information
Components
10Mobius 1000 Columns
10ClearSpin™ Filters
2ClearSpin Adapters
2 × 1 mlRNase A
2 × 100 mlBacterial Resuspension Buffer
2 × 100 mlBacterial Lysis Buffer
2 × 100 mlMobius Neutralization Buffer
2 × 125 mlMobius Equilibration Buffer
2 × 250 mlMobius Wash Buffer
2 × 65 mlMobius Elution Buffer
2 × 12.5 mlTE Buffer
Store and ship information
Storage +15°C to +30°C
Ship Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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