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The Mobius™ 1000 Plasmid Kits provide columns
and buffers for isolation of up to 1 mg ultrapure
plasmid DNA from 100 ml overnight bacterial
culture when isolating high-copy number
plasmids (Hendriks 2000). For low-copy number
plasmids, the kit can isolate up to 200 µg
DNA from a 250-ml culture, and up to 1 mg
from 500-ml to 1.5-L cultures using a scalable
protocol. The Mobius 1000 Kits incorporate a
novel ClearSpin filtration device that minimizes
the duration of centrifugation steps required for
the clarification of bacterial lysates.
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Product information
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Components
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| 10 rxn | 25 rxn |
| | • | 10 | 25 | Mobius 1000 Columns
| | • | 10 | 25 | ClearSpin Filters
| | • | 2 | 2 | ClearSpin Adapters
| | • | 1 ml | 2 × 1 ml | RNase A
| | • | 100 ml | 2 × 100 ml | Bacterial Resuspension Buffer
| | • | 100 ml | 2 × 100 ml | Bacterial Lysis Buffer
| | • | 100 ml | 2 × 100 ml | Mobius Neutralization Buffer
| | • | 125 ml | 2 × 125 ml | Mobius Equilibration Buffer
| | • | 250 ml | 2 × 250 ml | Mobius Wash Buffer
| | • | 65 ml | 2 × 65 ml | Mobius Elution Buffer
| | • | 12.5 ml | 2 × 12.5 ml | TE Buffer |
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Store and ship information
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Storage
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+15°C to +30°C
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Ship
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Shipped at Ambient Temperature or with Blue Ice
Multiple Toxicity Values, refer to MSDS
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