71220  MightyLight™ Rluc Assay Kit

The MightyLight™ Rluc Assay Kit is designed for sensitive promoter and enhancer analysis based on measurement of luciferase enzyme activity from Renilla reniformis. The bioluminescent properties of Renilla luciferase are well suited as a reporter enzyme because light emissions can be quantitatively captured in a photometer and light output directly correlated with the amount of enzyme present. Rluc assays take a matter of seconds as compared to other reporter systems that require lengthy incubations and tedious reagent additions.
The MightyLight Rluc Assay Kit is specifically designed to maximize reporter activity with maintenance of very low background. Coelentrazine substrate is provided as a stabilized 100X concentrate to be used in conjunction with the Rluc Assay Buffer. Sufficient reagent is provided for 100 assays of 100 µl each (equivalent to a 96-well plate). Provided for preparation of cellular extracts, Reportasol™ Extraction Buffer is composed of a buffered mixture of non-ionic and zwitterionic detergents designed for optimal preservation of Renilla luciferase activity. The pMLuc-1 Positive Control and pMLuc-3 CMV Positive Control are available separately to test assay performance and can serve as positive transfection controls. Applications

•	Extraction and detection of Renilla luciferase activity from mammalian cells
•	Designed for use with pMLuc-1, pMLuc-2, or pMLuc-3 AccepTor™ vectors or uncut vectors (available separately)