444146
MDM2, His•Tag®, Human, Recombinant, E. coli
Mouse Double Minute 2, Human, Recombinant, E. coli
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recombinant, human MDM2 expressed in E. coli and purified by affinity chromatography using FPLC. Useful for in vitro transcription assays and DNA-protein and protein interaction assays. MDM2 is an E3 ubiquitin ligase that was first identified in mouse as a small acentromeric extrachromosomal element. It was then shown to become oncogenic upon amplification and tumorigenic when over-expressed in NIH3T3 and Rat2 cells. MDM2 overexpression is known to promote tumor development by inhibiting wild-type p53 transcriptional activation.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 100 mM KCl, 20 mM Tris-Cl, 1 mM DTT, 0.2 mM EDTA, 20% glycerol, pH 8.0. | ||
| Molar mass | 54000 | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit is defined as 1 ng purified protein. | ||
| Biological activity | 20-200 ng is sufficient for an in vitro protease assay and 100 ng is sufficient for a protein-protein interaction assay. | ||
| Purity | > 95% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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