For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
24 May 2013
loading
| Source | Recombinant Thermococcus kodakaraensis KOD1 DNA polymerase expressed in E. coli (wild type and exonuclease-deficient forms) |
| Concentration | 2.5 U/µl |
| Endonuclease | None detected |
| Nicking activity | None detected |
| Amplification effiency | Functional PCR |
| Storage | –20°C |
*Manufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. Licensed under US Patent Number 5,436,149 owned by Takara Shuzo Co., Ltd.
Note: Purchase of this product includes an immunity from suit under patents specified in the product insert to use only the amount purchased for the purchaser's own internal research. No other patents rights (such as 5' Nuclease Process patent rights) are conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained by contacting the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California, 94404, USA.
| Product information | ||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Unit definition | One unit is defined as the amount of enzyme that will catalyze the incorporation of 10 nmol of dNTP into acid-insoluble form in 30 min at 75°C, in a reaction containing 20 mM Tris-HCl (pH 7.5 at 25°C), 8 mM MgCl2, 7.5 mM DTT, 50 µg/ml BSA, 150 µM each of dATP, dCTP, dGTP, dTTP (a mix of unlabeled and [3H]dTTP), and 150 µg/ml activated calf thymus DNA. | |||||||||||
| Components |
|
|||||||||||
| Features and benefits |
|
|||||||||||
| Declaration | Manufactured by Toyobo and distributed by Novagen. Not available from Novagen in Japan. Purchase of this product is accompanied by a limited license to use it in the Polymerase Chain Reaction (PCR) process for research use in conjunction with a thermal cycler whose use in the automated performance of the PCR process is covered by the up-front license fee, either by payment to Applied Biosystems or as purchased, i.e., an authorized thermal cycler. | |||||||||||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
||
