CBA044
K-LISA™ IKKβ Inhibitor Screening Kit
Inhibitor of nuclear factor kappa B kinase beta subunit screening kit, I-kappa-B-kinase beta screening kit, NFKBIKB screening kit, Nuclear factor NF-kappa-B inhibitor kinase beta screening kit, I-kappa-B kinase 2 screening kit, IKK2 screening kit, IκBKB screening kit
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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The K-LISA™ IKKβ-Inhibitor Screening Kit is designed for rapid in vitro screening of IKKβ inhibitors. The assay is an ELISA-based activity assay that utilizes a 50-amino acid GST-IκBα fusion polypeptide substrate that includes the Ser32 and Ser36 IKKβ phosphorylation sites. The GST-IκBα substrate and IKKβ are incubated in the presence of IKKβ inhibitors in the wells of a Glutathione-Coated 96-Well Plate, which allows for substrate phosphorylation and capture in a single step. The phosphorylated GST-IκBα substrate is detected using an Anti-Phospho IκBα (Ser32/Ser36) antibody, followed by the HRP-Conjugate and color development with TMB Substrate. ELISA Stop Solution is used to stop the color development and the absorbance is read at 450 nm preferably with a reference wavelength of 540-600 nm. The absorbance is directly related to the level of IKKβ activity.
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 96 Tests | ||
| Detection method | Colorimetric | ||
| Assay range | 5-10 ng | ||
| Sample type | Purified enzyme | ||
| Kit contains | Glutathione Coated 96-Well Plate, GST-IκBα (IKKβ Substrate), Anti-Phospho IκBα (Ser32/Ser36), IKKβ, Antibody Diluent, Kinase Assay Buffer, ATP/MgCl2 Mix, IKKβ Inhibitor 500X, Kinase Stop Solution, ELISA 20X Plate Wash Concentrate, HRP-Conjugate, TMB Substrate, ELISA Stop Solution, Kinase Stop Solution, Plate Sealers, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | Multiple storage requirements | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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