K-LISA™ Checkpoint Activity Kit
K-LISA™ Chk1/Chk2 Activity Kit
For general questions please contact our Customer Service:
290 Concord Road
01821 Billerica, MA
Phone: 781 533-6000
25 May 2013
• Highly versatile: can detect kinase activity in purified or partially purified Chk1 and Chk2 preparations.
• Highly sensitive: can detect kinase activity at picogram (Chk1) and nanogram levels (Chk2).
• Highly convenient: 96-well format, non-radioactive detection.
• Highly useful: enables high throughput screening of kinase inhibitors.
|CBA020||K-LISA™ Checkpoint Activity Kit|
|Accessories for K-LISA™ Checkpoint Activity Kit|
|Sensitivity||25 pg (0.018 mU) purified Chk1|
|Assay range||25-725 µU (Chk1) and 260 µU - 26 mU (Chk2)|
|Assay time||3 h|
|Sample type||Cell lysates, tissue extracts, purified enzymes|
|Kit contains||Biotinylated Chk1 Substrate, Phosphoserine Detector Antibody, Streptavidin Coated 96-Well Plate Strips, HRP-Conjugated Secondary Antibody, Serine/Threonine Kinase Assay Buffer, ATP/MgCl2, Chk1 Inhibitor, Wash Buffer, TMB Substrate, Stop Solution, Plate Sealers, and a user protocol. Purified Chk1 is available separately (Cat. No. 220479).|
|Store and ship information|
Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
Dry Ice Only
Multiple Toxicity Values, refer to MSDS
The activity of His•Tag®, Human, Recombinant Chk1 (Cat. No. 220479) (17-2200 pg) was determined using protocol A as indicated in the Detailed Protocol section. Assay range: 34 pg-1100 pg (740 Units/mg).
The activity of Human, Recombinant Chk2 (0.06-60 ng) was determined using protocol A in the Detailed Protocol section. Assay range: 200 pg-20 ng (1283 Units/mg).