70907  Introductory UltraMobius™ 1000 Plasmid Kit

The UltraMobius 1000 Plasmid Kits is designed for fast, convenient, and economical isolation of up to 1 mg ultra-low endotoxin plasmid DNA from 100 ml overnight bacterial culture, when isolating high-copy number plasmids (Hendriks 2000). For low-copy number plasmid, the kit can isolate up to 200 µg DNA from a 250-ml culture and up to 1 mg from 500-ml to 1.5-L cultures using a scalable protocol. The kits features the same high-capacity anion exchange tentacle resin as the other Mobius™ kits and incorporates a novel ClearSpin™ filtration device that minimizes the duration of centrifugation steps required for the clarification of lysates. The UltraMobius 1000 Kits include a Detox™ Agent to reduce endotoxin levels to below 20 EU/mg plasmid DNA. Purified plasmid DNA is suitable for the most demanding applications such as DNA microinjections and transfection of sensitive cell lines.