CBA025
InnoCyte™ ECM Cell Adhesion Assay, Laminin/Basement Membrane Complex (BMC)
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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The InnoCyte™ Cell Adhesion Assay, Laminin I/Basement Membrane Complex is designed for the determination of the relative attachment of adherent cell lines to laminin I and basement membrane protein complex, for evaluation of cell adhesion receptors, and for assessment of inhibitory or stimulatory substances for cell attachment. The kit is supplied with a 96-well strip plate coated with mouse laminin I and basement membrane complex (see plate format below). Cells are seeded in the coated wells and incubated at +37°C. Following incubation, the wells are washed briefly and attached cells are labeled the green fluorescent dye, calcein-AM. BSA-coated wells serve as a negative control and poly-L-lysine-coated wells serve as a positive control for general attachment. Relative cell attachment is assessed using a fluorescence plate reader.
• Convenient: 96-well plate format; allows cell attachment profiling of various cell lines at the same time
• Sensitive: cell-permeable fluorescent dye; no cell permeabilization needed
• Easy to use: does not require tedious staining method or expensive complicated equipment
• Convenient: 96-well plate format; allows cell attachment profiling of various cell lines at the same time
• Sensitive: cell-permeable fluorescent dye; no cell permeabilization needed
• Easy to use: does not require tedious staining method or expensive complicated equipment
| Product information | |||
|---|---|---|---|
| Format | 96-well plate | ||
| Form | 72 Tests (36 Laminin and 36 BMC) | ||
| Detection method | Fluorometric | ||
| Protect from Light | Yes | ||
| Avoid freeze/thaw | Yes | ||
| Assay time | 2.5 h | ||
| Sample type | Adherent cultured cells | ||
| Kit contains | Coated 96-Well Plates, Fluorescent Dye, D-PBS, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Data | |||
|---|---|---|---|
 ~40,000 cells were added to wells containing laminin I, BMC, or BSA and incubated for 1.5 h at 37°C in a cell culture incubator in the presence of 6% CO<sub>2</sub>. Cells were washed gently with D-PBS and labeled with Calcein-AM for 1 h at 37°C in a cell culture incubator in the presence of 6% CO<sub>2</sub>. Fluorescence was measured as indicated in the Detailed Protocol. HT-1080 cells displayed appreciable binding to poly-L-lysine and served as a positive control (data not shown). |
