For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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The GST•Tag™ Assay Kit is designed to perform quantitative colorimetric enzymatic assays of glutathione-S-transferase (GST) fusion proteins (Habig 1974). The kit is useful for the quantification of GST activity in crude samples or purified fractions. The suitability of this assay for crude samples allows expression conditions to be evaluated and rapidly optimized by comparing GST activity levels. The GST activity assay is simple to perform using the supplied 1-chloro-2,4-dinitrobenzene (CDNB) substrate. A sample is combined with CDNB substrate in the supplied reaction buffer and the absorbance of the reaction is monitored at 340 nm. The rate of change in A340 is proportionate to the amount of GST activity present in the sample. The assay has sufficient sensitivity to detect as little as eight pmol of functional GST, which corresponds to approximately 250 ng of unfused GST.
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| Avoid freeze/thaw | Yes | ||||||||||||||
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Multiple Toxicity Values, refer to MSDS |
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| S Phrase |
S: 7-16-26-36/37-45-28-60-61 Keep container tightly closed. Keep away from sources of ignition - No Smoking. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and gloves. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). This material and its container must be disposed of as hazardous waste. Avoid release to the environment. Refer to special instructions/safety data sheet. |
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| R Phrase |
R: 11-23/24/25-33-36/37/38-50/53 Highly flammable. Toxic by inhalation, in contact with skin and if swallowed. Danger of cumulative effects. Irritating to eyes, respiratory system and skin. Very toxic to aquatic organisms; may cause long-term adverse effects in the aquatic environment. |
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