For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
loading
Kindly contact Customer Service to order this product.
This product has been discontinued.
Please refer to our complete listing of Gateway® Protein Expression Vetors for possible alternatives. Please read all alternative product documentation carefully and contact technical service if you need additional information.
The Gateway Nova pET-54-DEST™ vector is designed to create expression clones (pEXPR) that are fused to an N-terminal His•Tag® coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target in the recombinant pEXPR clone will also be fused to a C-terminal S•Tag™ coding sequence. The S•Tag sequence allows quantification of the fusion protein using the FRETWorks™ assay (Cat. No. 70724). The S•Tag can also be used for purification and detection of the fusion protein. The expression kit contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation and recombination/expression, and the LR Clonase® II enzyme necessary for the Gateway recombination reaction.
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Irritant |
||
| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 22-26-36 Do not breathe dust. In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
||
| R Phrase |
R: 36/38 Irritating to eyes and skin. |
||
