71866  Gateway^® Nova pCOLA-3-DEST™ Expression System

This product has been discontinued.

Please refer to our complete listing of Gateway^® Protein Expression Vetors for possible alternatives. Please read all alternative product documentation carefully and contact technical service if you need additional information.

The Gateway Nova pCOLA-3-DEST™ vector carries the COLA replicon from ColA and the gene for kanamycin resistance. This DEST vector is compatible with pENTR vectors created using pDONR223, the pDONR vector used in the human ORFeome project. This DEST vector is NOT compatible with pENTR vectors derived from pDONR221 or other pENTR vectors carrying the kanR gene on their backbone. pEXPR recombinants created using pCOLA-3-DEST can be used for coexpression with expression plasmids generated from Gateway Nova pET-DEST vectors carrying the ColE1 replicon and AmpR gene. pCOLA-3-DEST is designed to create expression clones (pEXPR) that are fused to an N-terminal Strep•Tag^® II coding sequence. If the pENTR clone lacks a stop codon and is appropriately designed for a C-terminal fusion, the target gene in the resulting pEXPR clone will also be fused to a C-terminal His•Tag^® coding sequence. The expression system contains Nova F- Competent Cells for cloning, Rosetta™ 2 (DE3) Competent Cells for expression, control plasmids for transformation, recombination & expression, and the LR Clonase^® II enzyme for the recombination reaction.