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25 May 2012
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Recombinant, murine Dickkopf related protein-1 (Dkk-1), consisting of amino acids 30-272, fused at the C-terminus to a His•Tag® sequence, and expressed in NS0-dervied mouse myeloma cells. Dkk-1 is a well-documented antagonist of the Wnt signaling pathway. The balance between Dkk-1 inhibition and Wnt signaling is essential for bone formation and homeostasis.
| Product information | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Formulation | Lyophilized from 0.2 µm filtered PBS, BSA. | ||
| Molar mass | 27600 | ||
| Biological activity |
ND50: 0.2-1 µg/ml as measured by the inhibition of Wnt-3a-induced alkaline phosphatase production in MC3T3-E1 mouse osteoblastic cells. Reduction of Axis-inducing activity: 60-100% reduction (using 1 ng rmDkk-1 per embryo) in the number of secondary axes produced in the presence of 10-20 pg Xwnt-8 mRNA using early Xenopus embryos. |
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| Purity | ≥95% by SDS-PAGE | ||
| Contaminants | Endotoxin: ≤1.0 EU/µg protein | ||
| Solubility | PBS | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Regulatory Review |
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