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EMD Millipore
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United States
Phone: 781 533-6000
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25 May 2012
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Recombinant, human cytochrome P450 3A4 expressed in E. coli. The major heme-containing human liver P450 enzyme that is able to carry out a variety of oxidation, hydroxylation, and dealkylation reactions on a structurally diverse group of compounds. Suitable for in vitro drug metabolism and toxicology studies related to stereo- and/or region-specific hydroxylation reactions. Also suitable for use in reconstituted systems with purified NADPH-P450 oxidoreductase, cytochrome b5, and appropriate lipids.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 20 mM potassium phosphate buffer, 1 mM DTT, 200 µM EDTA, 20% (v/v) glycerol, pH 7.4. | ||
| Molar mass | 53000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | testosterone hydroxylation: ≥200 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will hydroxylate 1.0 nmol of testosterone per min at 37°C, pH 7.4. | ||
| Purity | ≥85% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
||
