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234401  Complement C1q-Depleted Serum, Human

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20 April 2014

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Product number Qty/Pk Quantity Price Available to Ship
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234401-1ML  1 ml 
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Human serum that has been depleted of C1q complement component.
Product information
Form Clear, amber solution
Formulation In PBS, ≤0.1% sodium azide, pH 7.4.
Preservative None
Avoid freeze/thaw Yes
Unit definition Classical Pathway Activity: One C1qH50 unit measured by classical pathway activation is defined as the amount of C1q required to yield 50% lysis of 3 x 107 EA when incubated in the presence of the recommended volume of C1q-Dpl for 30 min at 37°C in a total reaction volume of 500 µl GVB++. CH50 Functional Activity upon Reconstitution: One CH50 unit is defined as the input of C1q-Dpl, reconstituted with C1q, or NHS Complement Standard yielding 50% lysis of 1 x 108 EA when incubated for 60 min at 37°C in a total reaction volume of 1.5 ml GVB++. Alternative Pathway Activity: One unit of whole alternative pathway activity (APH50) is defined as the input of C1q-Dpl or NHS Complement Standard yielding 50% lysis of 1.5 x 107 rabbit erythrocytes when incubated for 30 min at 37°C in a total reaction volume of 75 ml GVB° containing a final Mg-EGTA concentration of 13.3 mM.
Contaminants C1q: No C1q detected by Ouchterlony using various dilutions of goat anti-human C1q antiserum.
Source Prepared from serum that has been shown by certified tests to be negative for HBsAg and for antibodies to HCV, HIV-I, and HIV-II.
Store and ship information
Storage ≤ -70°C
Ship Dry Ice Only
Standard Handling

© Merck KGaA, Darmstadt, Germany, 2014

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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