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69283  ColonyFinder® Immunoscreening Kit

Screen bacterial colonies expressing proteins of interest with monoclonal antibodies

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25 May 2012

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The ColonyFinder® Immunoscreening Kit is used to screen bacterial expression libraries with mouse monoclonal antibodies. Colony lifts are prepared as usual with nitrocellulose or PVDF membranes. The kit provides components for denaturing the colonies to expose cellular proteins, blocking buffers, Anti-Mouse IgG AP Conjugate and AP colorimetric detection reagents. Clones expressing epitopes recognized by mouse antibodies can be easily detected in several hours. A Positive Control Clone expressing the HSV•Tag® epitope and corresponding monoclonal antibody are also included to verify performance.

Product information
Avoid freeze/thaw Yes
Components
125 mlColony Denaturing Solution
40 ml10% Gelatin
35 ml20% Tween 20
40 µlGoat Anti-mouse IgG AP Conjugate
1.5 mlBCIP
1.5 mlNBT
20 ml20X AP Buffer
20 µlPositive Control Antibody
0.2 mlPositive Control Clone
Store and ship information
Storage Multiple storage requirements
Ship Blue Ice Only
Multiple Toxicity Values, refer to MSDS

© Merck KGaA, Darmstadt, Germany, 2012

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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