Information
X


70526  Clonables™ Ligation/Transformation Kit

Simple, reproducible ligation and transformation—in as little as 23 minutes

For general questions please contact our Customer Service:

EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com

Contact us


25 May 2012

loading
 
Product number Qty/Pk Quantity Price Delivery Date
      Please login to see your account specific pricing.
70526-3  11 rxn 
loading
 
Prices are subject to change without notice.
The Clonables™ Kit enables convenient, dependable, high-efficiency ligation and transformation of any compatible DNA ends. The kit features a unique, universal Ligation Premix, containing ligase, buffer, and cofactors, which supports ligation of any type of DNA cohesive or blunt ends in a 15-minute reaction. Ligated DNA is transformed into NovaBlue Singles™ Competent Cells, which use a streamlined protocol that takes less than 8 minutes for ampicillin-resistant plasmids and 38 minutes for other antibiotic-resistant plasmids. This kit can be used with a variety of cloning vectors and is compatible with any type of DNA end, without altering the ends or desired cloning junctions.

The Clonables Kit contains sufficient reagents to perform 11 ligation and transformation reactions, and includes a control vector and insert mix to verify performance.
Product information
Avoid freeze/thaw Yes
Components
55 µlClonables 2X Ligation Premix
10 µlClonables Positive Control
1.5 mlNuclease-free Water
11 × 50 µlNovaBlue Singles™ Competent Cells
2 × 2 mlSOC Medium
10 µl Test Plasmid
Features and benefits
  • Fast! 15-minute ligation, 8-minute transformation with ampicillin selection
  • Ligation components premixed to decrease pipetting steps and increase reliability
  • One reaction, optimized for cohesive ends, single base overhangs, and blunt ends
  • Single-use competent cells eliminate need to aliquot, freeze/thaw, or waste partially used vials
  • Compatible with PCR buffer, TE, restriction enzyme buffer, or End Conversion Mix
  • Store and ship information
    Storage ≤ -70°C
    Ship Dry Ice Only
    Multiple Toxicity Values, refer to MSDS

    © Merck KGaA, Darmstadt, Germany, 2012

    All references to Merck refer to Merck KGaA, Darmstadt, Germany.


    x

    Welcome to EMD Millipore

     

    Europe, Middle East and Africa Asia / Pacific North America, Central America and the Carribean South America

    Please select your country:

    North America, Central America and the Carribean

    South America

    Europe, Middle East and Africa

    Asia / Pacific

    All countries from A to Z

    If you can't find your country please click here.

     

    x

    EMD Millipore Chemicals Cart ()

    You are entering the Millipore online shop. Both carts are maintained for you while you shop between sites. We recommend to complete your Merck order before visiting Millipore.com.

    Go to Millipore Proceed to checkout


    What kind of products are you looking for?

    Millipore

    Bio manufacturing and Life science.

    EMD Millipore Chemicals

    High-quality industrial and laboratory chemicals.


    Important message


    It is currently not possible to order Bioscience products together with other EMD products. We recommend completing your current order first.

    Do you wish to proceed without completing your current order? If so, this will empty your shopping cart.