220487
Chk2, His•Tag®, Human, Recombinant, S. frugiperda
CHK2 checkpoint homolog, CDS1, RAD53, βA44G7, CHEK2
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EMD Millipore
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01821 Billerica, MA
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25 May 2012
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Recombinant, human checkpoint homologue kinase 2 (Chk2) fused to a His•Tag® sequence at the N-terminus and expressed in S. frugiperda insect cells. Chk2 is involved in sensing DNA damage in eukaryotic cells. It plays an important role in the control of the cell cycle by blocking replication.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 270 mM sucrose, 150 mM NaCl, 50 mM Tris-HCl, 1 mM DTT, 0.1 mM EGTA, pH 7.5. | ||
| Molar mass | 65430 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥ 25,000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme required to transfer 1 picomol phosphate to the synthetic peptide substrate KKKVSRSGLYRSPSMPENLNRPR per min at 30°C. | ||
| Purity | ≥ 95% by SDS PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Increasing amounts of Chk2, His•Tag<sup>®</sup>, Active, Human, Recombinant, <i>S. frugiperda</i> were assayed for activity as outlined above. |
