219459
Cdk5, GST-Fusion, Human, Recombinant, E. coli
Cyclin Dependent Kinase 5
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recombinant, human Cdk5 fused to GST and expressed in E. coli.. Cdk5 together with p35 (or p25; Cat. No. 480741), or its isoform, p39 (or p30; Cat. No. 480742), forms an active enzyme complex. The active enzyme phosphorylates a variety of substrates (i.e. tau, neurofilament, retinoblastoma protein, synapsin, and etc) that are involved in various cellular functions, including differentiation, migration, and apoptosis. Aberrant Cdk5 activity has been implicated in a number of neurodegenerative diseases such as Alzheimer’s disease, amyotrophic lateral sclerosis (ALS), and Parkinson’s disease, where abnormal phosphorylation of tau and/or neurofilaments has been observed. One vial contains sufficient material for approximately 25 reactions.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 25 mM Tris-HCl, 100 µM DTT, 30% Glycerol, pH 7.4. | ||
| Molar mass | 59000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥100,000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme, when reconstituted with either Nck5a (Cat. No. 480741) or Nck5ai (Cat. No. 480742), that will transfer 1.0 pmol phosphate to Histone H1 peptide (9-18) per min at 30°C, pH 7.2 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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