Caspase-1 Assay Kit, Fluorometric
For general questions please contact our Customer Service:
290 Concord Road
01821 Billerica, MA
Phone: 781 533-6000
24 May 2013
This product has been discontinued.
This kit provides a simple and convenient assay for measuring the activity of caspase-1 and related caspases that recognize the sequence YVAD. The assay is based on detection of the cleavage of the substrate YVAD-AFC. Upon cleavage of the substrate by caspase-1 or related caspases, free AFC emits a yellow-green fluorescence (~505 nm), which can be measured using a fluorometer or a fluorescence microplate reader.
|Protect from Light||Yes|
|Assay time||3-3.5 h|
|Sample type||cell lysates|
|Kit contains||Cell Lysis Buffer, Reaction Buffer, YVAD-AFC Substrate, DTT, and a user protocol.|
|Store and ship information|
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS
In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
Wear suitable protective clothing.
Wear eye/face protection.
Avoid release to the environment. Refer to special instructions/safety data sheet.
Harmful if swallowed.
Irritating to eyes and skin.
Risk of serious damage to eyes.
Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment.