For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
22 May 2013
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This kit provides a simple and convenient assay for measuring the activity of caspase-1 and related caspases that recognize the sequence YVAD. The assay is based on the spectrophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the substrate YVAD-pNA. pNA can be measured using a spectrophotometer or a microtiter plate reader at ~405 nm.
| Product information | |||
|---|---|---|---|
| Format | Cuvette or 96-well plate | ||
| Form | 100 Tests | ||
| Detection method | Colorimetric | ||
| Protect from Light | Yes | ||
| Assay time | 2.5-3 h | ||
| Sample type | Cell lysates | ||
| Kit contains | Cell Lysis Buffer, Reaction Buffer, YVAD-pNA Substrate, DTT, Dilution Buffer, and a user protocol. | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Ambient Temperature Only
Multiple Toxicity Values, refer to MSDS |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36/39-24-61 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing and eye/face protection. Avoid contact with skin. Avoid release to the environment. Refer to special instructions/safety data sheet. |
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| R Phrase |
R: 36/38-41-52/53 Irritating to eyes and skin. Risk of serious damage to eyes. Harmful to aquatic organisms, may cause long-term adverse effects in the aquatic environment. |
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