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EMD Millipore
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25 May 2012
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Recombinant, human casein kinase II expressed in E. coli. A cyclic nucleotide-independent serine/threonine protein kinase composed of catalytic α-subunits (M.W. 44 kDa) and regulatory β-subunits (M.W. 26 kDa) in an α2β2-tetramer. This material corresponds to one of several forms of native CKII. Suitable for in vitro phosphorylation of proteins and peptides, phosphopeptide synthesis, characterization of phosphorylating sites, screening of activators and inhibitors, and mechanistic studies.
Note: 1 KU = 1000 units.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 350 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, 0.1% TRITON® X-100 Detergent, pH 7.5. | ||
| Molar mass | 130000 | ||
| Avoid freeze/thaw | Yes | ||
| Unit definition | One unit is defined as the amount of enzyme that will catalyze the transfer of 1.0 pmol of phosphate to RRRADDSDDDDD per min at 30°C, pH 7.5, in a total volume of 25 µl. | ||
| Activity | ≥500,000 units/ml | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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