218709
Casein Kinase II, α2, Active, Human, Recombinant, E. coli
CK2α2, CSNK2A2
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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
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25 May 2012
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Recombinant, human casein kinase II, α2, (CKII, α2) fused to maltose binding protein (MBP) at the N-terminus and expressed in E. coli. CKII, α2 is a catalytic subunit of the serine/threonine protein kinase CKII. It is suitable for use in reactions where autophosphorylation of the regulatory CKII β-subunit is not desired. Shown to be extremely salt-sensitive with respect to its activity.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 200 mM NaCl, 20 mM Tris-HCl, 2 mM DTT, 1 mM EDTA, pH 7.4. | ||
| Molar mass | 84297 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥1 x 106 U/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme required to transfer 1 pmol phosphate to RRRDDDSDDD peptide substrate per min at 37°C. | ||
| Purity | ≥ 90% by SDS PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Increasing concentrations of Casein Kinase II, α2 were assayed according to the protocol outlined above. |
