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EMD Millipore
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Phone: 781 533-6000
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E-mail: Customer.CareDesk@emdchemicals.com
22 May 2013
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Native calpain-1 from human erythrocytes. Ca2+-dependent heterodimeric cysteine proteinase with low Ca2+ requirement (EC50= 2 µM).
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 20 mM imidazole, 5 mM β-mercaptoethanol, 1 mM EDTA, 1 mM EGTA, 30% glycerol, pH 6.8. | ||
| Molar mass | 112000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥1000 units/mg protein | ||
| Unit definition | One unit is defined as the amount of enzyme that will hydrolyze 1 pmol Suc-LLVY-AMC in 1 min, 25°C using the Calpain Activity Assay Kit, Fluorogenic (Cat. No. QIA120). Note: 1 caseinolytic unit = 1 fluorogenic unit. | ||
| Purity | Comparable to reference lot by SDS-PAGE | ||
| Source | Prepared from blood that has been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV. | ||
| EC number | 3.4.22.17 | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Harmful |
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| Safety information | |||
|---|---|---|---|
| S Phrase |
S: 26-36 In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. Wear suitable protective clothing. |
||
| R Phrase |
R: 36/37/38-42/43 Irritating to eyes, respiratory system and skin. May cause sensitization by inhalation and skin contact. |
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