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25 May 2012
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Calmodulin (CaM) purified from wheat germ (Triticum aestivum). Wheat germ calmodulin is unique in that it contains cysteine at position 26 (Cys26), which is in contrast to mammalian CaM that does not possess this residue. The single SH moiety on Cys26 makes this CaM preparation ideal for applications requiring both calmodulin activation and residue specific tagging. The sulfhydryl can be labeled using SH-reactive protein-modification reagents like fluorescent maleimide derivatives. Additionally, wheat germ CaM contains only a single tyrosine residue (Tyr138), compared to mammalian CaM that has two tyrosine residues, which can serve as a monospecific C-terminal labeling site.
Using Cys26 and Tyr138 allow wheat germ CaM to be independently derivatized at both the N- and C-termini making it a useful tool for structural, proteomic biomarker discovery and protein-protein interaction studies. Wheat germ CaM also contains no tryptophan residues, resulting in a distinctive absorbance spectrum when compared to mammalian CaM sequences.
| Product information | |||
|---|---|---|---|
| Form | Lyophilized | ||
| Formulation | Lyophilized from 10 mM sodium phosphate buffer, 5% sucrose, pH 7.0. | ||
| Molar mass | 16750 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥ 35,000 U/mg protein | ||
| Unit definition | One unit is defined as the amount of calmodulin that will stimulate 0.01 unit of 3'5'-cyclic nucleotide phosphodiesterase to 50% of the maximum activity of the enzyme when saturated with CaM in the presence of 100 µM Ca2+ in a 3 ml reaction volume at 30°C, pH 7.5. | ||
| Purity | >98% by SDS-PAGE | ||
| Solubility | H2O | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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