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EMD Millipore
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Phone: 781 533-6000
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E-mail: Customer.CareDesk@emdchemicals.com
23 May 2013
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BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)
DE3 indicates that the host is a lysogen of λDE3, and therefore carries a chromosomal copy of the T7 RNA polymerase gene under control of the lacUV5 promoter. Such strains are suitable for production of protein from target genes cloned in pET vectors by induction with IPTG.
pLysS strains express T7 lysozyme, which further suppresses basal expression of T7 RNA polymerase prior to induction, thus stabilizing pET recombinants encoding target proteins that affect cell growth and viability. Genotype: F- ompT hsdSB(rB- mB-) gal dcm (DE3) pLysS (CamR)
| Related products | |
|---|---|
| 70181 | NovaBlue Singles™ Competent Cells |
| 70235 | BL21(DE3) Singles™ Competent Cells |
| 70630 | Origami™(DE3) Singles™ Competent Cells |
| 71318 | NovaBlue T1R Singles™ Competent Cells |
| Related products for BL21(DE3)pLysS Singles™ Competent Cells | |
| 70181 | NovaBlue Singles™ Competent Cells |
| 70235 | BL21(DE3) Singles™ Competent Cells |
| 70630 | Origami™(DE3) Singles™ Competent Cells |
| 71318 | NovaBlue T1R Singles™ Competent Cells |
| 71400 | Rosetta™ 2(DE3) Singles™ Competent Cells |
| 71408 | Origami™ 2(DE3) Singles™ Competent Cells |
| Product information | ||||||||||||
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| Avoid freeze/thaw | Yes | |||||||||||
| Components |
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| Guaranteed efficiency | Guaranteed efficiency: > 2 x 107 cfu/µg | |||||||||||
| Store and ship information | |||
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| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Multiple Toxicity Values, refer to MSDS |
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| Licence Brookhaven | This product is covered under license from Brookhaven National Labs. Commercial entities need to obtain a research use license prior to purchase. Please contact your licensing department to confirm that your company already holds a research use license. | ||
