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EMD Millipore
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25 May 2012
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Recombinant, human ASK1 (accession no. NM_005923), consisting of amino acids 649-946, fused at the N-terminus to GST and expressed in S. frugiperda insect cells using a baculovirus expression system. ASK1, also known as MAPKKK5, activates MKK3, MKK4 (SEK1), and MKK6, is a serine/threonine kinase that is activated in response to TNFα. It plays a central role in stress-induced apoptosis; overexpression of ASK1 induces apoptotic cell death. The active kinase is useful in the study of ASK1 activity regulation and inhibitor screening.
| Product information | |||
|---|---|---|---|
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 50 mM Tris-HCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 25% glycerol, pH 7.5. | ||
| Molar mass | 60000 | ||
| Avoid freeze/thaw | Yes | ||
| Specific activity | ≥95 nmol/min/mg | ||
| Unit definition | Kinase activity is measured as the molar amount of phosphate incorporated into MBP at 30°C using a final concentration of 50 µM ATP. | ||
| Purity | ≥90% by SDS-PAGE | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | ≤ -70°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
  The specific activity of ASK1 was measured as outlined above. |
