For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
26 May 2013
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A two color TUNEL (Terminal deoxynucleotide transferase dUTP
Nick End Labeling) assay for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometry and microscopy. This kit labels the 3'-hydroxy termini of apoptotic DNA fragments with bromolated deoxyuridine triphosphate nucleotides (Br-dUTP). It is known that Br-dUTP is more readily incorporated into the genome of apoptotic cells compared to larger ligands, such as fluorescein or biotin.
| Product information | ||||
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| Format | Flow cytometry | |||
| Form | 60 Tests | |||
| Detection method | Fluorescence | |||
| Applications |
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| Avoid freeze/thaw | Yes | |||
| Components |
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| Assay time | 4-5 h | |||
| Sample type | Cells | |||
| Kit contains | Positive Control Cells, Negative Control Cells, Wash Buffer, Reaction Buffer, TdT, Br-dUTP, Rinsing Buffer, Fluorescein PRB-1 mAb, PI/RNase Staining Buffer, and a user protocol. | |||
| Store and ship information | |||
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Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping chargesmay be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges. |
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| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Multiple Toxicity Values, refer to MSDS |
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