CB1039
Anti-S100A4 Mouse mAb (1F12-1G7)
Anti-S100 Calcium Binding Protein A4
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EMD Millipore
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25 May 2012
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Recognizes the ~10 kDa S100A4 protein in HeLa and NIH3T3 cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | Full-length, recombinant, human S100A4 (aa 1-102), expressed as a GST fusion protein | ||
| Clone | 1F12-1G7 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human, mouse | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | HeLa cells, NIH3T3 cells | ||
| Negative control | 293T cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of human S100A4 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.  Detection of mouse S100A4 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human S100A4 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-S100A4 Mouse mAb (1F12-1G7) (Cat. No. CB1039) (10 µg/ml). Detection: fluorescence. |
