OP136
Anti-Rb (Ab-11) Mouse mAb (1F8)
Anti-Retinoblastoma Associated Protein, Anti-pp105
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EMD Millipore
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E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes the ~105 kDa Rb protein in the nuclei of BR-20 cells and primary human foreskin fibroblasts.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | a recombinant protein consisting of human retinoblastoma protein fused to β-galactosidase | ||
| Epitope | within amino acids 703-722 | ||
| Clone | 1F8 | ||
| Form | Liquid | ||
| Formulation | In 50 mM phosphate buffer, 50% glycerol. | ||
| Species reactivity | human, not mouse, not rat | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | ML-1, Molt-4, or U2OS cells or normal colon tissue | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human Rb by immunohistochemistry. Sample: Human breast carcinoma, tissue fixed in formalin and embedded in paraffin. Primary antibody: Anti-Rb (Ab-11) Mouse mAb (1F8) (Cat. No. OP136) (1 µg/ml). Detection: fluorescence.  Detection of human Rb by immunoblotting. Sample: Human Rb. Primary antibody: Anti-Rb (Ab-11) Mouse mAb (1F8) (Cat. No. OP136) (1 µg/ml). Detection: chemiluminescence. |
