AP1042
Anti-PTEN Mouse mAb (EMD-4B8)
Anti-TEP-1, Anti-MMAC, Anti-Phosphatase and Tensin Homologue Deleted on Chromosome Ten
For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes the ~55 kDa PTEN protein in MCF-7 cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | full-length, recombinant, human PTEN expressed in Sf9 cells | ||
| Clone | EMD-4B8 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | ≤ 0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | MCF7 or A431 cells | ||
| Negative control | MDA-MB-468 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Highly Toxic |
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| Data | |||
|---|---|---|---|
 Detection of human PTEN by immunoblotting. Samples: Whole cell lysate (20 µg) from MCF7 cells (Lane 1), A431 cells (Lane 2), and MDA-MB-48 cells (Lanes 3,4), and PTEN, His•Tag<sup>®</sup> and S•Tag™, Human, Recombinant, <i>S. frugiperda</i> (20 ng; Cat. No. 481409). Primary antibody: Anti-PTEN Mouse mAb (EMD-4B8) (Cat. No. AP1042) (2 µg/ml). Detection: colorimetric. |
