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EMD Millipore
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25 May 2012
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Recognizes the ~80 kDa PCSK1 protein in HeLa, PC12, Raw 264.7, and NIH3T3 cells and human small intestine tissue.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG3 | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids 652-754 of human PCSK1, expressed as a GST fusion protein | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | HeLa cells, PC12 cells, Raw 264.7 cells, NIH3T3 cells, or Human small intestine tissue | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of human PCSK1 by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.  Detection of rat PCSK1 by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.  Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from Raw 264.7 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.  Detection of mouse PCSK1 by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human PCSK1 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human small intestine tissue. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (3 µg/ml). Detection: colorimetric (DAB).  Detection of human PCSK1 by immunocytochemistry. Sample: HeLa cells fixed in paraformaldahyde. Primary antibody: Anti-PCSK1 Mouse mAb (Cat. No. ST1537) (30 µg/ml). Detection: fluorescence. |
