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EMD Millipore
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United States
Phone: 781 533-6000
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E-mail: Customer.CareDesk@emdchemicals.com
20 May 2013
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Recognizes the ~55-60 kDa OCLN protein in HeLa cells and human and mouse liver tissue.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG | ||
| Immunogen | Full-length, human OCLN (aa 1-522) | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human, mouse | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Human liver tissue, Mouse liver tissue, HeLa cells | ||
| Negative control | 293T cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of human OCLN by immunoblotting. Sample: Whole tissue extract from human liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.  Detection of mouse OCLN by immunoblotting. Sample: Whole tissue extract from mouse liver. Primary antibody: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human OCLN by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-OCLN Mouse pAb (Cat. No. CB1045) (1:1200) and an anti-CSNK1E mouse mAb (1:50). Detection: fluorescence using Duolink® 30 Detection Kit 613 (Red) and DAPI (blue) counterstain. |
