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EMD Millipore
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Phone: 781 533-6000
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25 May 2012
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Recognizes the ~50 kDa HTT protein in U-2 OS and HeLa cells and human salivary gland tissue.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG2a | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids 81-191 of human HTT, expressed as a GST fusion protein | ||
| Clone | 3F1 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | U-2 OS cells, HeLa NE cells, Human salivary gland tissue | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of human HTT by immunoblotting. Sample: Whole cell lysate from U-2 OS cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human HTT by immunoblotting. Sample: Whole cell lysate from HeLe NE cells. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human HTT by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human salivary gland tissue. Primary antibody: Anti-HTT Mouse mAb (3F1) (Cat. No. ST1529) (3 µg/ml). Detection: colorimetric (DAB). |
