For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
loading
Recognizes the ~70 kDa HSPA1B protein in HeLa, PC-12, IMR-32, Raw 264.7, and NIH3T3 cells and human testis tissue.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG2a | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids 531-612 of human HSPA1B, expressed as a GST fusion protein | ||
| Clone | 3B7 | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | None | ||
| Applications |
|
||
| Avoid freeze/thaw | Yes | ||
| Positive control | PC12 cells, IMR-32 cells, Raw 264.7 cells, NIH3T3 cells, Human testis tissue, HeLa cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
||
| Data | |||
|---|---|---|---|
 Detection of rat HSPA1B by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human HSPA1B by immunoblotting. Sample: Whole cell lysate from IMR-32 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.  Detection of mouse HSPA1B by immunoblotting. Sample: Whole cell lysate from NIH3T3 cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (1-5 µg/ml). Detection: chemiluminescence.  Detection of rat HSPA1B by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human testes. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (6 µg/ml). Detection: Colorimetric (DAB).  Detection of rat HSPA1B by immunocytochemistry. Sample: HeLa cells. Primary antibody: Anti-HSPA1B Mouse mAb (3B7) (Cat. No. CA1039) (10 µg/ml). Detection: fluorescence. |
