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EMD Millipore
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25 May 2012
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Recognizes the ~15 kDa H2AX protein and the ~24 kDa monoubiquinated form in 293T and A431 cell nuclear extracts.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide corresponding to amino acids at the C-terminus of human H2AX | ||
| Form | Liquid | ||
| Formulation | In Tris/citrate-phosphate buffer, pH 7-8. | ||
| Species reactivity | human, mouse | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Positive control | Nuclear extracts from human 293T or G-361 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | +2°C to +8°C | ||
| Ship |
Blue Ice Only
Highly Toxic |
||
| Data | |||
|---|---|---|---|
 Detection of human H2AX by immunoblotting. Sample: Nuclear extract from HEK293T cells, H2AX knockout (-/-), H2AX wild-type (+/+), and G-361 cells. Primary antibody: Anti-H2AX Rabbit pAb (Cat. No. DR1016) (1:1000). Detection: chemiluminescence. |
