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EMD Millipore
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E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes the ~40-45 kDa E2F2 protein in HeLa cells and mouse kidney tissue.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | Full-length, human E2F2 (aa 1-437) | ||
| Form | Liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human, mouse | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Mouse kidney tissue, HeLa cells | ||
| Negative control | 293T cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of mouse E2F2 by immunoblotting. Sample: Whole tissue extract from mouse kidney. Primary antibody: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human E2F2 by immunocytochemistry using a proximity ligation assay (protein-protein interaction). Sample: HeLa cells. Primary antibodies: Anti-E2F2 Rabbit pAb (Cat. No. DR1095) (1:1200) and an Anti-E2F2 Mouse mAb (1:50). Detection: fluorescence using Duolink<sup>®</sup> 30 Detection Kit 613 (Red) and DAPI (blue) counterstain. |
