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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes the ~15 kDa cytochrome c protein in P3588 D1, HeLa, Jurkat, and NIH3T3 cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | rat cytochrome c | ||
| Clone | 6H2.B4 | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | P388D1, Hela, Jurkat, or NIH/3T3 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Irritant |
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| Data | |||
|---|---|---|---|
 Lane 1: Detection of human cytochrome <i>c</i> by immunoblotting. Samples: Whole cell lysate (50 μg) from MCF7 cells. Primary antibodies: Anti-Cytochrome <i>c</i> Mouse mAb (7H8.2C12) (Cat. No. AP1029) (1 μg/ml). Detection: chemiluminescence. Lanes 2,3: Detection of human cytochrome <i>c</i> by immunoprecipitation followed by immunoblotting. Sample: Whole cell lysate (500 μg) from MCF7 cells. Antibody for immunoprecipitation: Anti-Cytochrome <i>c</i> Mouse mAb (7H8.2C12) (Cat. No. AP1029) (10 μg/ml) (lane 2) and Anti-Cytochrome <i>c</i> Mouse mAb (6H2.B4) (10 μg/ml) (lane 3). Immunoblotting conditions: same as lane 1.  Detection of human cytochrome <i>c</i> by immunocytochemistry. Sample: HeLa cells fixed with 100% methanol. Primary antibody: Anti-Cytochrome <i>c</i> Mouse mAb (6H2.B4) (Cat. No. AP1030) (3 μg/ml). Detection: fluorescence with DAPI counterstain. |
