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AP1013  Anti-Cleaved Caspase-8 (Asp384) Mouse mAb (11G10)

Anti-Cleaved CAP4 (Asp384), Anti-Cleaved CASP8 (Asp384), Anti-Cleaved FLICE (Asp384), Anti-Cleaved ICE-LAP5 (Asp384), Anti-Cleaved MACH (Asp384), Anti-Cleaved Mch5 (Asp384)

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21 May 2013

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Recognizes the ~10 kDa active subunit of caspase-8. Does not cross-react with full length caspase-8.
Product information
Host Mouse
Isotype IgG1
Immunogen a synthetic peptide corresponding to amino acids near the N-terminus of the p10 subunit of human caspase-8
Clone 11G10
Form Liquid
Formulation In 150 mM NaCl, 10 mM sodium HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5.
Species reactivity human
Preservative ≤0.1% sodium azide
Applications
Immunoblotting (Western Blotting)
Avoid freeze/thaw Yes
Positive control Anti-fas treated SKW6.4 cells
Store and ship information
Storage -20°C
Ship Blue Ice Only
Standard Handling
Data

Detection of human Cleaved Caspase-8 by immunoblotting. Samples: Whole cell extracts from SKW6.4 cells, untreated or treated with anti-Fas. Primary antibody: Anti-Cleaved Caspase-8 (Asp384) Mouse mAb (11G10) (Cat. No. AP1013) (1:1000). Detection: chemiluminescence.

© Merck KGaA, Darmstadt, Germany, 2013

All references to Merck refer to Merck KGaA, Darmstadt, Germany.


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