For general questions please contact our Customer Service:
EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
21 May 2013
loading
Kindly contact Customer Service to order this product.
Recognizes the ~17-19 kDa large fragment of cleaved caspase-3. Does not recognize full-length caspase-3 or other cleaved caspases.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide corresponding to amino acids adjacent to the Asp¹75 cleavage site of human caspase-3 | ||
| Form | Liquid | ||
| Formulation | In 150 mM NaCl, 10 mM HEPES, 100 µg/ml BSA, 50% glycerol, pH 7.5. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | None | ||
| Applications |
|
||
| Positive control | HeLa, NIH3T3, or C6 cells treated with staurosporine (Cat. No. 569397) | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
||
| Data | |||
|---|---|---|---|
 Detection of human, mouse, and rat cleaved caspase-3 by immunoblotting. Samples: Whole cell lysates from untreated, staurosporine-treated (Cat. No. 569397; 1 µM), or cytochrome c-treated (250 µg/ml) HeLa, NIH 3T3, and C6 cells. Primary antibodies: Anti-Caspase-3 (upper) and Anti-Cleaved Caspase-3 (Asp175) Rabbit pAb (Cat. No. AP1027) (1:1000) (lower). Detection: chemiluminescence. |
