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EMD Millipore
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25 May 2012
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Recognizes the ~63 kDa (apparent MW) calreticulin protein in Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198, and vero cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | a recombinant protein consisting of human calreticulin fused to maltose binding protein | ||
| Clone | FMC75 | ||
| Form | Liquid | ||
| Formulation | In PBS, 0.1 mM PMSF, 50% glycerol, pH 7.2. | ||
| Species reactivity | not bovine, not canine, not E. coli, not guinea pig, not hamster, human, monkey, not mouse, not rat, not salmon | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | Human Molt 4, MCF-7, HeLa, K562, A431, Jurkat, Hs67, Hs198 cells, or monkey Vero cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human calreticulin by immunoblotting. Samples: Whole cell lysate from HeLa cells treated by heat shock (lane 1) and Vero cells (lane 2). Primary antibody: Anti-Calreticulin Mouse mAb (FMC75) (Cat. No. 208912) (1:1000). Detection: chemiluminescence. |
