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EMD Millipore
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Phone: 781 533-6000
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E-mail: Customer.CareDesk@emdchemicals.com
19 May 2013
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Recognizes the ~400 kDa BRCA2 protein in MCF-7 and HeLa cells and breast carcinoma.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a recombinant protein consisting of amino acids 3245-3418 of human BRCA2 | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human | ||
| Preservative | ≤ 0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | MCF-7 and HeLa cells and breast carcinoma | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human BRCA2 by immunoprecipitation and immunoblotting. Samples: Whole cell lysate from MCF-7 cells (750 µg) (lower panel) and HeLa cells (750 µg) (upper panel). Antibody for IP: Rabbit IgG Control (5µg, lane 1) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (5 µg, lane 2; 10 µg, lane 3; and 15 µg, lane 4). Conditions for immunoblotting: Samples: Whole cell lysate (100 µg) from MCF-7 cells and HeLa cells (lane 5, lower and upper panels, respectively) and BRCA2 IP samples (lanes 1-4). Primary antibody: Anti-BRCA2 (Ab-1) Mouse mAb (2B) (Cat. No. OP95) (2 µg/ml). Detection: chemiluminescence.  Detection of human BRCA2 by staining paraffin sections. Sample: Breast carcinoma tissue (formalin-fixed, paraffin-embedded, heat-pretreated in 10 mM sodium citrate buffer). Primary antibody: Rabbit IgG Control (10 µg/ml, left panel) and Anti-BRCA2 Rabbit pAb (Cat. No. CA1033) (10 µg/ml, right panel). Detection: DAB with methyl green counterstain. |
