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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
24 May 2013
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Recognizes the ~23 kDa Bim protein in K562 and A549 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide [(C)AERPPQLRPGAPTSLQTEP] corresponding to amino acids 22-40 of human Bim, conjugated to KLH | ||
| Form | Liquid | ||
| Formulation | In PBS. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | K562 or A549 cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human Bim by immunoblotting. Samples: Whole cell lysate from K562 cells at primary antibody dilutions 2.5 µg/ml (lane A) and µg/ml (lane B). Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (5 µg/ml). Detection: chemiluminescence.  Detection of human Bim by immunocytochemistry. Sample: K562 cells. Primary antibody: Anti-Bim (22-40) Rabbit pAb (Cat. No. 202000) (10 µg/ml). Detection: fluorescence. |
