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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 800-645-5476
Fax: 800-645-5439
E-mail: Customer.CareDesk@emdchemicals.com
19 June 2013
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Recognizes the ~21 kDa Bcl-w protein in 3T3 cells.
| Product information | |||
|---|---|---|---|
| Host | Rabbit | ||
| Isotype | IgG | ||
| Immunogen | a synthetic peptide (DFVGYKLRQKGYVC) corresponding to amino acids 16-29 of human Bcl-w, conjugated to KLH | ||
| Form | Liquid | ||
| Formulation | In 100 mM boric acid, 75 mM NaCl, 25 mM sodium borate, 5 mM EDTA, pH 8.2. | ||
| Species reactivity | bovine, human, monkey, mouse, rat | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Dry Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of mouse Bcl-w by immunoblotting. Samples: Whole cell lysate from untransfected 293T cells (lane A), cells tansfedted with mouse Bcl-w, 293T and NIH/3T3 cells (lane C). Primary antibody: Anti-Bcl-w (16-29) Rabbit pAb (Cat. No. 197209) (1:1000). Detection: chemiluminescence.  Detection of mouse Bcl-w by immunofluorescence. Sample: Methanol-fixed NIH/3T3 cells showing cytoplasmic localization. Primary antibody: Anti-Bcl-w (16-29) Rabbit pAb (Cat. No. 197209) (1:500). Detection: fluorescence. |
