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EMD Millipore
290 Concord Road
01821 Billerica, MA
United States
Phone: 781 533-6000
Fax: +49 6151 72 2000
E-mail: Customer.CareDesk@emdchemicals.com
25 May 2012
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Recognizes the ~102 kDa α-catenin protein in HeLa cells.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG1 | ||
| Immunogen | full-length, recombinant human α-catenin, expressed in E. coli | ||
| Clone | 1G5 | ||
| Form | Liquid | ||
| Formulation | Undiluted ascites. | ||
| Species reactivity | human | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Standard Handling |
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| Data | |||
|---|---|---|---|
 Lane 1: Detection of human α-catenin by immunoblotting. Sample: Whole cell lysate (25 µg) from HeLa cells. Primary antibody: Anti-α-Catenin Mouse mAb (1G5) (Cat. No. CA1030) (1:1000). Detection: chemiluminescence. Lane 2: Detection of human α-catenin by immunoprecipitation followed by immunoblotting. Antibody for immunoprecipitation: Anti-α-Catenin Mouse mAb (1G5) (Cat. No. CA1030) (1:100/500 µg total protein). Immunoblotting conditions: same as lane 1. |
