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EMD Millipore
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20 May 2013
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Recognizes the ~60 kDa Akt protein in A431 cells. Useful for the concentration of Akt in samples by immunoprecipitation prior to use in the K-LISA™ Akt Activity Kit (Cat. No. CBA019).
Antibody Target Gene Symbol: AKT1
Target Synonym: AKT, MGC99656, PKB, PKB-ALPHA, PKB/AKT, PRKBA, Protein kinase B, RAC, RAC-ALPHA, Thymoma viral proto-oncogene 1
Entrez Gene Name: v-akt murine thymoma viral oncogene homolog 1
Hu Entrez ID: 207 (Related Antibodies: 530311, PK1203, PC510 and 124001)
Mu Entrez ID: 11651
Rat Entrez ID: 24185
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG | ||
| Immunogen | a recombinant protein consisting of amino acids 1-149 of human Akt 1 fused to GST | ||
| Clone | SKB1 | ||
| Form | Liquid | ||
| Formulation | 100 µg antibody in 150 mM NaCl, 100 mM Tris-glycine, 30% glycerol, pH 7.4 and 100 µg control lysate. | ||
| Species reactivity | human, mouse, rat | ||
| Preservative | ≤0.1% sodium azide | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | A431 cells (included) | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Shipped with Blue Ice or with Dry Ice
Standard Handling |
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| Data | |||
|---|---|---|---|
 Detection of human Akt by immunoblotting. Sample: Whole cell lysate from A431 cells. Primary antibody: Anti-Akt, PH Domain Mouse mAb (SKB1) (Cat. No. ST1088) (0.5 µg/ml). Detection: chemiluminescence. |
