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25 May 2012
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Recognizes the ~37 kDa AKR1B10 protein in HepG2 and HeLa cells and human colon tissue.
| Product information | |||
|---|---|---|---|
| Host | Mouse | ||
| Isotype | IgG2a | ||
| Immunogen | A recombinant polypeptide corresponding to amino acids 76-144 of human AKR1B10, expressed as a GST fusion protein | ||
| Clone | 1A6 | ||
| Form | liquid | ||
| Formulation | In PBS, pH 7.2. | ||
| Species reactivity | human | ||
| Preservative | None | ||
| Applications |
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| Avoid freeze/thaw | Yes | ||
| Positive control | HepG2 cells, Human Colon tissue, HeLa cells | ||
| Store and ship information | |||
|---|---|---|---|
| Storage | -20°C | ||
| Ship |
Blue Ice Only
Regulatory Review |
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| Data | |||
|---|---|---|---|
 Detection of human AKR1B10 by immunoblotting. Sample: Whole cell lysate from HepG2 cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (1-5 µg/ml). Detection: chemiluminescence.  Detection of human AKR1B10 by staining paraffin sections. Sample: Formalin-fixed, paraffin-embedded human colon tissue. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (3 µg/ml). Detection: colorimetric (DAB).  Detection of human AKR1B10 by immunocytochemistry. Sample: Paraformaldahyde treated HeLa cells. Primary antibody: Anti-AKR1B10 Mouse mAb (1A6) (Cat. No. ST1507) (10 µg/ml). Detection: fluorescence. |
